Main sol. J238
Internal Solution-ID: S3916
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| CaSO4 x 2 H2O | 1.01 | g | 1.01 | 5.866 | |
| Ca(NO3)2 x 4 H2O | 0.49 | g | 0.49 | 2.075 | |
| MgSO4 x 7 H2O | 0.70 | g | 0.7 | 2.84 | |
| K2SO4 | 25.00 | mg | 0.025 | 0.143 | |
| K2HPO4 | 5.00 | mg | 0.005 | 0.029 | |
| NaHCO3 | 0.20 | g | 0.2 | 2.381 | |
| FeCl3 x 6 H2O | 1.00 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Glucose | 10.00 | mg | 0.01 | 0.056 | |
|
Soil extract
(see below) |
50.00 | ml | - | - | |
| Agar | 15.00 | g | 15 | 44.599 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Adjust pH to 6.6-6.8. | |||||
| 2 To prepare soil extract, equal volumes of leafy soil and tap water are boiled for 2 hr and then cleared by centrifugation. | |||||
Media with this solution
| J238 | ARTIFICIAL SOIL AGAR |