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Main sol. J239

Internal Solution-ID: S3917

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Asparagine 1 g 90.909 688.08
Glycerol 2 g 181.818 1974.35
K2HPO4 1 g 90.909 521.938
Polypeptone
(Nihon Pharm. Co.)
30 g 2727.27 -
Trace salt solution 1 ml - -
Vitamin B solution 10 ml - -
Agar 15 g 1363.64 4054.46
1 Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.
J239 JCM MEDIUM No. 239