Main sol. J274
Internal Solution-ID: S3965
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
2.00 | g | 2 | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 2 | 28.137 | |
| Sodium tartrate | 1.00 | g | 1 | 5.153 | |
| Trisodium citrate | 1.00 | g | 1 | 3.875 | |
| NaNO3 | 0.25 | g | 0.25 | 2.941 | |
| (NH4)2SO4 | 0.25 | g | 0.25 | 1.892 | |
| Ferric citrate | 0.20 | g | 0.2 | 0.817 | |
| MJ(-N) synthetic seawater | 100.00 | ml | - | - | |
| Na2SO4 | 1.00 | g | 1 | 7.04 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 2 | 8.058 | |
| Na2CO3 | 40.00 | g | 40 | 377.401 | |
| Distilled water | 900.00 | ml | - | - | |
| 1 Adjust pH to 11.0-12.0 with KOH and autoclave under a N2 atmosphere. After autoclaving, replace the gas phase with N2-CO2 (80:20) and pressurize to 200 kPa. | |||||
Media with this solution
| J274 | MJ/YTCT MEDIUM |