Main sol. J277
Internal Solution-ID: S3969
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 2 | - | |
| NaNO3 | 1.70 | g | 1.7 | 20.001 | |
| FeSO4 x 7 H2O | 1.39 | g | 1.39 | 5 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| MJ(-N) synthetic seawater | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except NaNO3, FeSO4 x 7H2O and Na2S x 9H2O, and autoclave under a N2 atmosphere. Separately autoclave NaNO3, FeSO4 x 7H2O and Na2S x 9H2O solutions (pH 7.0, 7.5 and 7.5, respectively) under a N2 atmosphere. Aseptically and anaerobically add the NaNO3, FeSO4 x 7H2O and Na2S x 9H2O solutions to the medium and adjust pH to 6.0. Pressurize the inoculated bottle to 200 kPa N2. | |||||
Media with this solution
| J277 | MJYP* MEDIUM |