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Main sol. 2

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Internal Solution-ID: S4

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 1 1000 ml - -
Urea 20 g/l 20 333.023
1 To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea.
2 For sporulation enhancement add 10 mg/l MnSO4 x H2O.
2 BACILLUS PASTEURII MEDIUM