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Main sol. 2
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Internal Solution-ID: S4
Equipment needed:
Autoclave
Compound
Amount
Unit
Conc. [g/L]
Conc. [mM]
Medium 1
1000
ml
-
-
Urea
20
g/l
20
333.023
1
To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea.
2
For sporulation enhancement add 10 mg/l MnSO
4
x H
2
O.
Media with this solution
2
BACILLUS PASTEURII MEDIUM
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