Main sol. J344
Internal Solution-ID: S4064
This solution is used in 2 media and was first defined in medium J344.
Internal Solution-ID: S4064
This solution is used in 2 media and was first defined in medium J344.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
MJ(-N) synthetic seawater | 670.00 | ml | - | - | |
NH4Cl | 0.25 | g | 0.245 | 4.573 | |
NaNO3 | 1.00 | g | 0.978 | 11.512 | |
Na2WO4 x 2 H2O | 0.10 | mg | 9.8e-5 | - | |
Distilled water | 330.00 | ml | - | - | |
1 Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized): | |||||
NaHCO3
(8%) |
12.50 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
2 Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). | |||||
3 Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water. |