Main sol. J347
Internal Solution-ID: S4067
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Synthetic seawater (2 x)
(2 x) |
500.0 | ml | - | - | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| (NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
|
Yeast extract
(BD-Difco) |
0.4 | g | 0.4 | - | |
|
Sulfur
(powder) |
10.0 | g | 10 | 311.818 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 500.0 | ml | - | - | |
| 1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, and autoclave. Separately autoclave 10% (w/v) yeast extract solution, and 5% Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, add the yeast extract solution, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture. | |||||
Media with this solution
| J347 | SME MEDIUM |