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Main sol. J356

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Internal Solution-ID: S4075

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
MJ(-N) synthetic seawater 1000.00 ml - -
NH4Cl 0.25 g 0.25 4.669
NaHCO3 1.50 g 1.499 17.838
Na2S2O3 x 5 H2O 1.50 g 1.499 6.038
Trace vitamins 1.00 ml - -
1 Mix ingredients, except NaHCO3, Na2S2O3 x 5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3 x 5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.
J356 MJ BASAL MEDIUM