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Poorly crystalline iron(III) oxide

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Internal Solution-ID: S4143

This solution is used in 4 media and was first defined in medium J411.

Equipment needed: Centrifuge
1 Dissolve 108 g FeCl3 in 1.0 L distilled water. Adjust pH to 7.0 with 10 N NaOH. Allow the suspension to sit for 30 min, then readjust pH to 7.0. Centrifuge the suspension at 5,000 x g for 20 min and pour off supernatant. Wash the precipitate with distilled water by resuspending in distilled water followed by the centrifugation. Repeat the washing at least 5 times, then, resuspend in 1.0 L distilled water. Use the Fe(III) suspension as 0.67 M solution. More precisely, check the concentration of Cl- to be less than 1 mM and determine the concentration of Fe(III) by using hydroxylamine as described by Lovley and Phillips (Appl. Environ. Microbiol. 54, 751-757, 1986).
J411 Fe(III)-REDUCER MARINE ENRICHMENT MEDIUM
J412 Fe(III)-REDUCER FRESHWATER ENRICHMENT MEDIUM
J425 A1 MARINE MEDIUM
J665 FRESHWATER IRON GEL MEDIUM