Main sol. J412
Internal Solution-ID: S4145
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Poorly crystalline iron(III) oxide | 100.000 | mM | - | - | |
| MgCl2 x 6 H2O | 0.330 | g | 16.5 | 81.16 | |
| CaCl2 x 2 H2O | 0.330 | g | 16.5 | 112.233 | |
| KCl | 0.330 | g | 16.5 | 221.328 | |
| KH2PO4 | 0.330 | g | 16.5 | 121.247 | |
| NH4Cl | 0.250 | g | 12.5 | 233.689 | |
| NaHCO3 | 2.500 | g | 125 | 1487.97 | |
| Na2SeO4 | 18.900 | mg | 0.945 | 5.001 | |
| Trace vitamins | 10.000 | ml | - | - | |
| Trace mineral solution | 10.000 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.044 | g | 2.2 | - | |
| FeCl2 x 4 H2O | 0.260 | g | 13 | 65.388 | |
| 1 Add components except L-cysteine x HCl x H2O and FeCl2 x 4H2O to distilled water, bring volume to 1.0 L and adjust pH to 7.0. Distribute the medium into appropriate culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, sparge with the same gas mixture for 10 - 15 min, seal with butyl rubber stoppers and autoclave. Separately autoclave concentrated (100- or 200-fold) L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions under a N2 gas atmosphere. Prior to inoculation, add the sterile L-cysteine x HCl x H2O and FeCl2 x 4H2O solutions. Pressurize the inoculated vessels to 100 kPa H2-CO2 (4:1, v/v) and cultivate without shaking. | |||||
| 2 Comment: For strain JCM 12379, inoculate with a 10% inoculum. | |||||
Media with this solution
| J412 | Fe(III)-REDUCER FRESHWATER ENRICHMENT MEDIUM |