Main sol. J417
Internal Solution-ID: S4151
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.00 | g | 19.782 | 338.508 | |
K2HPO4 | 0.90 | g | 0.89 | 5.111 | |
KH2PO4 | 0.70 | g | 0.692 | 5.088 | |
CaCl2 x 2 H2O | 1.00 | g | 0.989 | 6.728 | |
NH4Cl | 1.25 | g | 1.236 | 23.115 | |
MgSO4 x 7 H2O | 3.40 | g | 3.363 | 13.644 | |
MgCl2 x 6 H2O | 4.18 | g | 4.135 | 20.337 | |
KCl | 0.33 | g | 0.326 | 4.378 | |
NiCl2 x 6 H2O | 5.00 | mg | 0.005 | 0.038 | |
Na2SeO3 x 5 H2O | 5.00 | mg | 0.005 | 0.019 | |
Fe2(SO4)3 x n H2O | 0.01 | g | 0.01 | - | |
H2WO4 | 5.00 | mg | 0.005 | 0.02 | |
FeSO4 x 7 H2O | 0.02 | g | 0.02 | 0.071 | |
Yeast extract
(BD-Difco) |
1.00 | g | 0.989 | - | |
Tryptone
(BD-Difco) |
1.00 | g | 0.989 | 13.916 | |
Sodium formate | 0.20 | g | 0.198 | 2.909 | |
Trace mineral solution | 10.00 | ml | - | - | |
Trace vitamins | 1.00 | ml | - | - | |
NaHCO3 | 2.00 | g | 1.978 | 23.549 | |
Sulfur
(powder) |
10.00 | g | 9.891 | 308.425 | |
Na2S x 9 H2O | 0.50 | g | 0.495 | 2.059 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix ingredients, except Trace vitamins, NaHCO3 and sulfur, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sterilized sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, sparge for 5 min with the same gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add the sterilized Na2S x 9H2O solution to the medium, and pressurize to 200 kPa H2-CO2 (4:1, v/v). Readjust pH to 5.5, if necessary. |
J417 | MJYTFSH MEDIUM |