Main sol. J439
Internal Solution-ID: S4185
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 0.955 | 16.343 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.382 | 1.879 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.096 | 0.65 | |
| NH4Cl | 0.25 | g | 0.239 | 4.464 | |
| KH2PO4 | 2.00 | g | 1.91 | 14.037 | |
| KCl | 0.50 | g | 0.478 | 6.406 | |
| Yeast extract | 0.50 | g | 0.478 | - | |
| Na2SO4 | 4.00 | g | 3.82 | 26.897 | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.6e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0. Bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
Sodium lactate
(1.0 M) |
20.00 | ml | - | - | |
|
Vitamin solution
(*) |
1.00 | ml | - | - | |
|
Thiamine solution
(*) |
1.00 | ml | - | - | |
|
Vitamin B12 solution 0.05 g/L
(*) |
1.00 | ml | - | - | |
|
NaHCO3
(*, 8.0%) |
20.00 | ml | - | - | |
| 2 Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
2.00 | ml | - | - | |
Media with this solution
| J439 | DESULFOVIBRIO AEROTOLERANS DVO5 MEDIUM |