Main sol. J446
Internal Solution-ID: S4193
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 3.00 | g | 2.97 | 50.826 | |
MgCl2 x 6 H2O | 0.34 | g | 0.337 | 1.656 | |
MgSO4 x 7 H2O | 0.42 | g | 0.416 | 1.687 | |
KCl | 0.05 | g | 0.05 | 0.664 | |
K2HPO4 | 0.14 | g | 0.139 | 0.796 | |
NH4Cl | 0.25 | g | 0.248 | 4.628 | |
CaCl2 x 2 H2O | 0.07 | g | 0.069 | 0.471 | |
Trace mineral solution | 10.00 | ml | - | - | |
Na2S2O3 x 5 H2O | 1.00 | g | 0.99 | 3.989 | |
Sulfur
(powder) |
3.00 | g | 2.97 | 92.619 | |
NaHCO3 | 1.00 | g | 0.99 | 11.786 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix ingredients except NaHCO3 and sulfur, adjust pH to 7.5 with NaOH and autoclave. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.06 g sulfur) under a stream of CO2-O2 (93:7, v/v) gas mixture, seal with butyl rubber stoppers and readjust pH to 7.5 with NaOH. Pressurize the inoculated vessel to 100 kPa CO2-O2 (93:7, v/v). |
J446 | MJTSO MEDIUM |