Main sol. J457
Internal Solution-ID: S4204
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 195.00 | g | - | - | |
| MgCl2 x 6 H2O | 35.00 | g | - | - | |
| MgSO4 x 7 H2O | 50.00 | g | - | - | |
| KCl | 5.00 | g | - | - | |
| NaHCO3 | 0.25 | g | - | - | |
| NaNO3 | 1.00 | g | - | - | |
| CaCl2 x 2 H2O | 0.50 | g | - | - | |
| KH2PO4 | 0.05 | g | - | - | |
| NH4Cl | 0.03 | g | - | - | |
| Yeast extract | 0.05 | g | - | - | |
| Sodium pyruvate | 1.00 | g | - | - | |
| 1 Add components except NaHCO3 and sodium pyruvate to distilled water and bring volume to 1.0 L. Adjust pH to 7.4 with 1 M Tris base and autoclave. Filter-sterilize 8% (w/v) NaHCO3 and 25% (w/v) sodium pyruvate solutions and add to the medium aseptically. | |||||
Media with this solution
| J457 | ARTIFICIAL SEAWATER FOR HALOPHILES |