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Main sol. J476

Internal Solution-ID: S4227

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
D-Glucose 10 g 10 55.506
KH2PO4 10 g 10 73.483
Peptone 5 g 5 -
Yeast extract 5 g 5 -
NaCl 50 g 50 855.578
Distilled water 1000 ml - -
1 Mix components and autoclave. After cooling down to room temperature, adjust pH to 9.0 with 20% (w/v) Na2CO3 (sterilized by autoclaving).
J476 BACILLUS OKHENSIS MEDIUM