Main sol. J478
Internal Solution-ID: S4229
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 19.782 | 338.508 | |
| K2HPO4 | 0.90 | g | 0.89 | 5.111 | |
| KH2PO4 | 0.70 | g | 0.692 | 5.088 | |
| CaCl2 x 2 H2O | 0.80 | g | 0.791 | 5.382 | |
| NH4Cl | 0.25 | g | 0.247 | 4.623 | |
| NaNO3 | 1.00 | g | 0.989 | 11.637 | |
| MgSO4 x 7 H2O | 4.00 | g | 3.956 | 16.052 | |
| MgCl2 x 6 H2O | 3.00 | g | 2.967 | 14.596 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| NiCl2 x 6 H2O | 1.00 | mg | 9.9e-4 | 0.008 | |
| Na2SeO3 x 5 H2O | 1.00 | mg | 9.9e-4 | 0.004 | |
| Fe2(SO4)3 x n H2O | 0.01 | g | 0.01 | - | |
| H2WO4 | 1.00 | mg | 9.9e-4 | 0.004 | |
| Na2S2O3 x 5 H2O | 2.00 | g | 1.978 | 7.971 | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Trace vitamins | 1.00 | ml | - | - | |
| NaHCO3 | 2.00 | g | 1.978 | 23.549 | |
|
Sulfur
(powder) |
10.00 | g | 9.891 | 308.425 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients except Trace vitamins, NaHCO3 and sulfur, adjust pH to 6.8 and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.2 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v). | |||||
Media with this solution
| J478 | MODIFIED MMJHS MEDIUM FOR GO25 |