Co-factors solution
Internal Solution-ID: S4340
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Pyridoxal hydrochloride | 250.0 | mg | 0.25 | - | |
| Pyridoxal phosphate | 250.0 | mg | 0.25 | 1.012 | |
| Ca-folinate | 50.0 | mg | 0.05 | - | |
| beta-NAD | 50.0 | mg | 0.05 | 0.075 | |
| Coenzyme A | 50.0 | mg | 0.05 | 0.065 | |
| FAD | 50.0 | mg | 0.05 | 0.064 | |
| Nicotinamide | 25.0 | mg | 0.025 | 0.205 | |
| Folic acid | 2.5 | mg | 0.003 | 0.006 | |
| Riboflavin | 0.5 | mg | 5.0e-4 | 0.001 | |
|
Hemin
(dissolved separately in 10 mM NaOH) |
65.0 | mg | 0.065 | 0.1 | |
| Thiamine pyrophosphate | 2.5 | g | 2.5 | 5.426 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0 with 1 N NaOH and filter-sterilize. | |||||
Media with this solution
| J537 | TREPONEMA ISOPTEROCOLA MEDIUM |