Solution A
Internal Solution-ID: S4345
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution | 50.0 | ml | - | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Vitamin solution | 5.0 | ml | - | - | |
| Na2SO4 | 2.8 | g | 3.491 | 24.58 | |
| Resazurin | 1.0 | mg | 0.001 | 0.005 | |
| Distilled water | 745.0 | ml | - | - | |
| 1 Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into culture vessels (e.g., 8 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Media with this solution
| J540 | MODIFIED SYNTROPHOMONAS MEDIUM FOR OL4 |