Main sol. 207
Internal Solution-ID: S440
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| Na2HPO4 x 12 H2O | 2.1 | g | 2.1 | 14.793 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Trace element solution | 10.0 | ml | - | - | |
| FeSO4 x 7 H2O | 1.5 | mg | 0.002 | 0.005 | |
| Wolin's vitamin solution | 5.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| NaHCO3 | 4.0 | g | 4 | 47.615 | |
| Yeast extract | 2.0 | g | 2 | - | |
| Na-lactate | 5.0 | g | 5 | 44.619 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl. | |||||
Media with this solution
| 207 | PROPIONISPIRA MEDIUM |