Main sol. 210
Internal Solution-ID: S442
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 130.0 | g | 121.495 | 2078.98 | |
| MgSO4 x 7 H2O | 8.8 | g | 8.224 | 33.368 | |
| KCl | 1.0 | g | 0.935 | 12.536 | |
| Yeast extract | 10.0 | g | 9.346 | - | |
|
Trypticase
(BBL) |
10.0 | g | 9.346 | - | |
| Wolin's vitamin solution | 10.0 | ml | - | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions: | |||||
| Thioglycolate-ascorbate solution | 25.0 | ml | - | - | |
|
Glucose
(10 % w/v) |
25.0 | ml | 2.5 | 13.877 | |
|
NaOH
(2 N) |
10.0 | ml | - | - | |
| 2 Final pH: 9.0. | |||||
Media with this solution
| 210 | HALOANAEROBIUM MEDIUM |