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Solution C

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Internal Solution-ID: S4459

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2 g 40 476.151
Distilled water 50 ml - -
1 Mix components, filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture.
2 To complete the medium, combine Solutions A, B, C and 1 ml Vitamin solution (see below) aseptically under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into sterilized culture vessels under the same gas mixture and seal with butyl rubber stoppers. Then, add 0.01 volume of 5% Na2S x 9H2O solution (autoclaved under a N2 atmosphere). Check the final pH to be 7.0 - 7.2. Pressurize the inoculated vessels to 70 kPa N2-CO2 (4:1, v/v) overpressure.
J602 DESULFOVIBRIO MAGNUS MEDIUM