Main sol. J621
Internal Solution-ID: S4483
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| HEPES | 4.76 | g | 4.667 | 19.582 | |
| NaCl | 20.00 | g | 19.608 | 335.521 | |
| Na2SO4 | 4.00 | g | 3.922 | 27.609 | |
| MgCl2 x 6 H2O | 4.30 | g | 4.216 | 20.736 | |
| NH4Cl | 0.25 | g | 0.245 | 4.582 | |
| KCl | 0.34 | g | 0.333 | 4.471 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.137 | 0.934 | |
| K2HPO4 | 0.14 | g | 0.137 | 0.788 | |
| Yeast extract | 1.00 | g | 0.98 | - | |
|
Sodium lactate
(50%) |
4.50 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks: | |||||
| Trace elements | 1.00 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%) |
1.00 | ml | - | - | |
| Trace vitamins | 10.00 | ml | - | - | |
|
Na2S x 9 H2O
(0.5 M) |
4.00 | ml | - | - | |
| 2 Adjust pH to 7.0. | |||||
Media with this solution
| J621 | HYL MEDIUM |