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Trace elements

Internal Solution-ID: S4484

This solution is used in 3 mediaand was first defined in medium J621.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 29.00 g 29 -
MnSO4 x n H2O 3.26 g 3.26 -
CoCl2 x 6 H2O 1.80 g 1.8 13.863
ZnSO4 x 7 H2O 1.00 g 1 3.478
NiSO4 x 6 H2O 0.11 g 0.11 0.711
CuSO4 x 5 H2O 0.10 g 0.1 0.401
H3BO3 0.10 g 0.1 1.617
AlK(SO4)2 x 12 H2O 0.10 g 0.1 -
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO3 0.05 g 0.05 -
Distilled water 1000.00 ml - -
1 First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.
J621 HYL MEDIUM
J764 HEPES PEPTONE-YEAST EXTRACT MEDIUM
J1066 M25Y MEDIUM