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FeS solution

Internal Solution-ID: S4499

This solution is used in 2 media and was first defined in medium J628.

Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 46.2 g 154 553.927
Na2S x 9 H2O 39.6 g 132 549.583
Distilled water 300.0 ml - -
1 Prewarm distilled water at 50C in a beaker with a stir bar. While rapidly starring the water, add FeSO4 x 7H2O, followed immediately by Na2S x 9H2O. A thick black precipitate will form instantly. Stir continuously for 2 to 3 min. Remove the mixture to a narrow-mouthed glass bottle (500 ml) that can be tightly stopped. The bottle is filled to the top with distilled water and capped. Allow to settle for several hours, decant and replace the overlaying water. Repeat this procedure at least five times to wash the FeS precipitate. Check pH to be close to neutral. To stock the FeS solution, fill the bottle with distilled water and cap. Remove the overlaying water when prepare the bottom layer.
J628 FRESHWATER IRON-OXIDIZING BACTERIA MEDIUM
J1067 FRESHWATER IRON-OXIDIZING BACTERIA MEDIUM-II