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Mix components except L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Bring to a boil for 5-10 sec and cool down under a stream of N2-CO2 (80:20, v/v). Distribute into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave L-cysteine x HCl x H2O and Na2S x 9H2O as 5% solutions under a N2 atmosphere. Prior to inoculation, add L-cysteine x HCl x H2O and Na2S x 9H2O solutions anaerobically.
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