Main sol. J671
Internal Solution-ID: S4561
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 3.00 | g | 2.93 | - | |
|
Tryptone
(BD-Difco) |
10.00 | g | 9.766 | 137.389 | |
| NH4Cl | 0.90 | g | 0.879 | 16.431 | |
| NaCl | 0.90 | g | 0.879 | 15.04 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.391 | 1.921 | |
| KH2PO4 | 0.75 | g | 0.732 | 5.382 | |
| K2HPO4 | 1.50 | g | 1.465 | 8.41 | |
| Trace element solution | 9.00 | ml | - | - | |
| Trace vitamins | 5.00 | ml | - | - | |
| FeSO4 x 7 H2O | 3.00 | mg | 0.003 | 0.011 | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Mix ingredients and adjust pH to 7.0. Distribute the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave the following solutions under a N2 atmosphere and add to the medium (per 950 ml): | |||||
|
Glucose
(10%) |
50.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
| 2 Readjust pH of the medium to 7.2 - 7.4 with sterile NaOH, if necessary. | |||||
Media with this solution
| J671 | THERMOANAEROBIUM MEDIUM |