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Trace mineral solution

Internal Solution-ID: S4585

This solution is used in 5 media and was first defined in medium J684.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 12.50 g 12.5 65.397
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 -
CoCl2 x 6 H2O 0.02 g 0.02 0.154
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.20 g 0.2 1.488
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.02 g 0.02 0.076
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals.
J684 ANAEROBIC TYEG MEDIUM
J715 USAB 49 MEDIUM
J716 USAB 53 MEDIUM
J717 TYE MEDIUM FOR STRAIN AeB
J718 SPOROLITUUS THERMOPHILUS MEDIUM