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Solution C

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Internal Solution-ID: S4661

Equipment needed: Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 4 g 57.143 680.215
Distilled water 50 ml - -
1 To complete the medium, combine Solutions A, B and C, and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Finally, add the following solutions from anaerobic stocks:
L-Cysteine HCl x H2O
(3%) 		10 ml - -
Na2S x 9 H2O
(3%) 		10 ml - -
2 Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
3 Adjust pH of the medium to 7.0, if necessary.
J731 SPOROMUSA MEDIUM