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Trace element solution

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Internal Solution-ID: S4676

This solution is used in 2 media and was first defined in medium J741.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 10.00 mg 0.01 0.04
AlK(SO4)2 x 12 H2O 20.00 mg 0.02 -
H3BO3 10.00 mg 0.01 0.162
Na2MoO4 x 2 H2O 10.00 mg 0.01 0.041
NiCl2 x 6 H2O 25.00 mg 0.025 0.193
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
2 Adjust final pH of the medium to 7.0 with KOH.
J741 ACETOBACTERIUM MEDIUM
J967 KD-1 MEDIUM