Main sol. J764
Internal Solution-ID: S4704
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| HEPES | 2.38 | g | 2.352 | 9.869 | |
| NaCl | 30.00 | g | 29.644 | 507.26 | |
| MgSO4 x 7 H2O | 2.70 | g | 2.668 | 10.825 | |
| MgCl2 x 6 H2O | 4.30 | g | 4.249 | 20.9 | |
| NH4Cl | 0.25 | g | 0.247 | 4.618 | |
| KCl | 0.50 | g | 0.494 | 6.627 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.941 | |
| K2HPO4 | 0.14 | g | 0.138 | 0.794 | |
|
Bacto peptone
(BD-Difco) |
2.00 | g | 1.976 | - | |
| Yeast extract | 0.20 | g | 0.198 | - | |
| Trace elements | 1.00 | ml | - | - | |
|
Fe(NH4)2(SO4)2 x 6 H2O
(in 0.037% HCl, 0.2%) |
1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.25. Autoclave and after cooling, add the following solution (filter-sterilized): | |||||
| Trace vitamins | 10.00 | ml | - | - | |
Media with this solution
| J764 | HEPES PEPTONE-YEAST EXTRACT MEDIUM |