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Main sol. J782

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Internal Solution-ID: S4731

Equipment needed: Filter Autoclave Anaerobic workbench Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.284 2.09
K2HPO4 0.30 g 0.284 1.633
NH4Cl 1.00 g 0.948 17.721
KNO3 1.70 g 1.611 15.938
NaCl 1.00 g 0.948 16.22
KCl 0.10 g 0.095 1.271
CaCl2 x 2 H2O 0.10 g 0.095 0.645
MgCl2 x 6 H2O 0.25 g 0.237 1.166
Trace minerals 10.00 ml - -
Yeast extract 1.00 g 0.948 -
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 7.2 with KOH and distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		25.00 ml - -
Glucose
(1 M) 		20.00 ml - -
J782 CALDINITRATIRUPTOR MICROAEROPHILUS MEDIUM