Main sol. J785
Internal Solution-ID: S4734
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| 1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.3 | g | 0.28 | 5.242 | |
| KH2PO4 | 0.3 | g | 0.28 | 2.06 | |
| KCl | 0.5 | g | 0.467 | 6.268 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.093 | 0.636 | |
| NaCl | 15.0 | g | 14.019 | 239.882 | |
| KNO3 | 1.5 | g | 1.402 | 13.866 | |
| Yeast extract | 0.5 | g | 0.467 | - | |
| Trace minerals | 10.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
MgCl2 x 6 H2O
(10%) |
5.0 | ml | - | - | |
|
Glucose
(1 M) |
20.0 | ml | - | - | |
| Trace vitamins | 10.0 | ml | - | - | |
Media with this solution
| J785 | NAD S1 MEDIUM |