Main sol. J786
Internal Solution-ID: S4735
Equipment needed: Autoclave
Anaerobic workbench
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.282 | 2.074 | |
| K2HPO4 | 0.3 | g | 0.282 | 1.62 | |
| NH4Cl | 1.0 | g | 0.941 | 17.587 | |
| NaCl | 1.0 | g | 0.941 | 16.097 | |
| MgCl2 x 6 H2O | 1.0 | g | 0.941 | 4.627 | |
| KCl | 0.1 | g | 0.094 | 1.262 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.094 | 0.64 | |
| Trace minerals | 10.0 | ml | - | - | |
| Yeast extract | 2.0 | g | 1.881 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.47 | - | |
| Resazurin | 1.0 | mg | 9.4e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.0 with KOH, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
|
Glucose
(1 M) |
20.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - | |
Media with this solution
| J786 | CLOSTRIDIUM LIND MEDIUM |