25 mM Ti(III) NTA solution
Internal Solution-ID: S4788
Equipment needed: Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 9.6 | g | 32 | 167.417 | |
|
Water
(anaerobic ) |
300.0 | ml | - | - | |
| 1 To prepare anaerobic water, boil 1.0 L distilled water and cool down under a stream of oxygen-free N2 gas. Mix components under N2 gas. Adjust pH to 9.0 with NaOH. Slowly add 9.6 ml of 20% TiCl3 to the chelator solution. Keep the pH above 2.0 by the addition of anaerobic saturated Na2CO3 solution. Adjust pH to 7.0 with Na2CO3 solution, adjust pH to 7.0 and bring the final volume to 500 ml with the anaerobic water. Filter-sterilize and keep the solution under a N2 gas atmosphere. | |||||
Media with this solution
| J827 | DEHALOCOCCOIDES MEDIUM |