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Main sol. J828

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Internal Solution-ID: S4789

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1.0 g 0.985 3.997
CaCl2 x 2 H2O 0.2 g 0.197 1.34
KNO3 1.0 g 0.985 9.745
Trace element solution SL-4 5.0 ml - -
Chelated iron solution 10.0 ml - -
Distilled water 900.0 ml - -
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
Phosphate buffer solution 100.0 ml - -
2 Adjust pH to 6.8, if necessary. Aseptically distribute the medium into sterile serum bottles (leave &; 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.
J828 MODIFIED NMS MEDIUM