Main sol. J830
Internal Solution-ID: S4793
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 1.00 | g | 0.999 | 18.676 | |
KH2PO4 | 0.30 | g | 0.3 | 2.202 | |
K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
Na2SO4 | 2.80 | g | 2.797 | 19.693 | |
NaCl | 20.00 | g | 19.98 | 341.889 | |
KCl | 0.10 | g | 0.1 | 1.34 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
Na2S2O3 x 5 H2O | 3.16 | g | 3.157 | 12.72 | |
Yeast extract | 0.10 | g | 0.1 | - | |
Trace element solution | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
Distilled water | 900.00 | ml | - | - | |
1 To complete the medium, aseptically and anaerobically add the following stock solutions (per liter, final volume): | |||||
Sodium lactate
(1.0 M) |
20.00 | ml | - | - | |
MgCl2 x 6 H2O
(15%) |
20.00 | ml | - | - | |
NaHCO3
(5%) |
40.00 | ml | - | - | |
Na2S x 9 H2O
(2%) |
20.00 | ml | - | - | |
2 Mix ingredients and adjust pH to 7.4. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture tubes under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
3 Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere. | |||||
4 Readjust pH of the medium to 7.4, if necessary. |
J830 | DESULFOVIBRIO B0109P2 MEDIUM |