Main sol. J843
Internal Solution-ID: S4807
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.297 | 2.185 | |
K2HPO4 | 0.30 | g | 0.297 | 1.707 | |
NH4Cl | 1.00 | g | 0.991 | 18.528 | |
NaCl | 20.00 | g | 19.822 | 339.179 | |
KCl | 0.10 | g | 0.099 | 1.329 | |
CaCl2 x 2 H2O | 0.10 | g | 0.099 | 0.674 | |
Trace element solution | 1.00 | ml | - | - | |
Yeast extract | 2.00 | g | 1.982 | - | |
Na2S2O3 x 5 H2O | 4.96 | g | 4.916 | 19.807 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.496 | - | |
Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
Distilled water | 925.00 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
MgCl2 x 6 H2O
(10%) |
30.00 | ml | - | - | |
Sodium acetate
(1.0 M) |
20.00 | ml | - | - | |
2 Then, aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere): | |||||
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
3 Pressurize inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). |
J843 | LIND BHT MEDIUM |