Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.05 | g | 0.05 | - | |
| K2HPO4 | 0.25 | g | 0.25 | 1.435 | |
| FeSO4 x 7 H2O | 0.01 | g | 0.01 | 0.036 | |
| Na2MoO4 x 2 H2O | 1.00 | mg | 1.0e-3 | 0.004 | |
| MnSO4 x H2O | 2.00 | mg | 0.002 | 0.012 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| NaCl | 0.10 | g | 0.1 | 1.711 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| Biotin | 0.10 | mg | 1.0e-4 | 4.1e-4 | |
| Agar , if required | 1.50 | % | 15 | 44.599 | |
| Distilled water | 950.00 | ml | - | - | |
|
Glucose
(filter-sterilized, 20 %) |
25.00 | ml | 5 | 27.753 | |
|
Na malate
(filter-sterilized, 20 %) |
25.00 | ml | 5 | 28.082 | |
| 1 Adjust pH of the medium to 7.1. Add 1.5% agar if needed. Autoclave for 15 min at 121°C. After sterilization add 25 ml each of filter-sterilized 20% glucose and 20% Na-malate. | |||||
Media with this solution
| 221 | AZOSPIRILLUM MEDIUM |