Main sol. J847
Internal Solution-ID: S4813
Equipment needed: Filter
Autoclave
Anaerobic workbench
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.282 | 2.074 | |
| K2HPO4 | 0.30 | g | 0.282 | 1.62 | |
| NH4Cl | 0.50 | g | 0.47 | 8.794 | |
| NaCl | 1.00 | g | 0.941 | 16.097 | |
| KCl | 0.10 | g | 0.094 | 1.262 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.094 | 0.64 | |
| MgCl2 x 6 H2O | 0.06 | g | 0.056 | 0.278 | |
| Trace minerals | 10.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.941 | - | |
| Resazurin | 1.00 | mg | 9.4e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.5 with KOH. Distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
Glucose
(1.0 M) |
20.00 | ml | - | - | |
|
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Media with this solution
| J847 | C5BEL MEDIUM |