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Solution D

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Internal Solution-ID: S4833

Equipment needed: Centrifuge Filter
1 Dilute (1:1, w/v) the content of terminal pig colon with the following buffer: 167 ml Solution A (see above) and 167 ml Solution B (see above). Make up to 1.0 L with distilled water. Stomach the suspension and filter through a cheese cloth. Incubate at 37C for 48 h and adjust pH to 7.0. Centrifuge at 20,000 x g for 20 min. Retrieve the supernatant and store at -20C until use.
J854 COLON EXTRACT MEDIUM