Main sol. J868
Internal Solution-ID: S4854
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.3 | g | 0.298 | 2.189 | |
K2HPO4 | 0.3 | g | 0.298 | 1.71 | |
NH4Cl | 1.0 | g | 0.993 | 18.565 | |
NaCl | 1.0 | g | 0.993 | 16.993 | |
KCl | 0.1 | g | 0.099 | 1.332 | |
CaCl2 x 2 H2O | 0.1 | g | 0.099 | 0.675 | |
Na2SO4 | 3.0 | g | 2.979 | 20.974 | |
Sodium acetate | 1.0 | g | 0.993 | 12.106 | |
Trace element solution | 1.0 | ml | - | - | |
Yeast extract | 1.0 | g | 0.993 | - | |
L-Cysteine HCl x H2O | 0.5 | g | 0.497 | - | |
Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
Distilled water | 950.0 | ml | - | - | |
1 Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 950 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
NaHCO3
(*, 8%) |
25.0 | ml | - | - | |
Fructose
(1 M) |
20.0 | ml | - | - | |
Vitamin solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
Thiamine solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
Vitamin B12 solution (see Medium No. 403)
(*) |
1.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
8.0 | ml | - | - |
J868 | GEOMONAS SACCHAROVORANS MEDIUM |