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Main sol. 226

Internal Solution-ID: S486

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casamino acids 2.0 g 2 -
Yeast extract 1.0 g 1 -
Glucose 1.0 g 1 5.551
(NH4) citrate 1.0 g 1 -
K2HPO4 2.0 g 2 11.483
MgSO4 x 7 H2O 0.5 g 0.5 2.029
FeCl3 x 6 H2O 10.0 mg 0.01 0.037
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0.
2 After sterilization, aseptically add 1 ml/l of a 10% solution of acetylacetone in ethanol.
226 AUREOBACTERIUM TERREGENS MEDIUM