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Main sol. J935

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Internal Solution-ID: S4947

Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.315 5.892
KH2PO4 0.33 g 0.315 2.316
KCl 0.33 g 0.315 4.228
CaCl2 x 2 H2O 0.44 g 0.42 2.859
MgCl2 x 6 H2O 0.70 g 0.669 3.289
NaCl 0.50 g 0.478 8.172
NaHCO3 0.80 g 0.764 9.096
FeCl2 solution 1.00 ml - -
Trace element solution 1.00 ml - -
Resazurin 1.00 mg 9.6e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components thoroughly, adjust pH to 5.0 and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):

(w/v) Yeast extract (BD-Difco, 10%) 		5.00 ml - -

(w/v) Trypticase peptone (BD-BBL, 10%) 		20.00 ml - -
Trace vitamins
(*) 		10.00 ml - -
Na2S x 9 H2O
(w/v, 5%) 		10.00 ml - -
2 Readjust pH to 5.5 - 6.0 with sterile 1 N H2SO4, if necessary.
J935 FERVIDICOCCUS FONTIS MEDIUM