Main sol. J947
Internal Solution-ID: S4969
Equipment needed: Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.899 | 16.809 | |
| NaCl | 25.00 | g | 24.975 | 427.362 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.966 | |
| KH2PO4 | 0.75 | g | 0.749 | 5.506 | |
| K2HPO4 | 1.50 | g | 1.499 | 8.603 | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.998 | - | |
| Yeast extract | 1.00 | g | 0.999 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| FeCl3 x 6 H2O | 2.50 | mg | 0.002 | 0.009 | |
| Cellobiose | 1.00 | g | 0.999 | 2.919 | |
| Glucose | 1.00 | g | 0.999 | 5.545 | |
| L-Cysteine HCl x H2O | 0.75 | g | 0.749 | - | |
| Resazurin | 0.50 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.5. | |||||
| 2 Prepare medium anaerobically under 100% N2. Add cellobiose after autoclaving from an anoxic stock solution prepared under N2 gas atmosphere and sterilized by filtration. | |||||
Media with this solution
| J947 | CALDICELLULOSIRUPTOR MEDIUM WITH 2.5% NaCl |