Main sol. J950
Internal Solution-ID: S4972
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 1.0 | g | 0.999 | 7.033 | |
| NaCl | 30.0 | g | 29.97 | 512.834 | |
| KCl | 0.7 | g | 0.699 | 9.38 | |
| MgCl2 x 6 H2O | 5.0 | g | 4.995 | 24.569 | |
| CaCl2 x 2 H2O | 1.0 | g | 0.999 | 6.795 | |
| K2HPO4 | 0.4 | g | 0.4 | 2.294 | |
| KH2PO4 | 0.4 | g | 0.4 | 2.936 | |
| NH4Cl | 0.5 | g | 0.5 | 9.338 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 2.0 | mg | 0.002 | - | |
|
Yeast extract
(BD-Difco) |
3.0 | g | 2.997 | - | |
|
Tryptone
(BD-Difco) |
4.0 | g | 3.996 | 56.218 | |
| Sodium acetate | 6.8 | g | 6.793 | 82.814 | |
| L-Cysteine HCl x H2O | 0.6 | g | 0.599 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 6.8. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solution (per 960 ml) from anaerobic stock (filter-sterilized) | |||||
|
NaHCO3
(5%) |
40.0 | ml | - | - | |
Media with this solution
| J950 | NH-JN4 MEDIUM |