Main sol. J953
Internal Solution-ID: S4975
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.285 | 2.092 | |
| K2HPO4 | 0.30 | g | 0.285 | 1.634 | |
| NH4Cl | 1.00 | g | 0.949 | 17.737 | |
| NaCl | 2.00 | g | 1.898 | 32.47 | |
| KCl | 0.10 | g | 0.095 | 1.273 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.095 | 0.645 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.474 | 2.333 | |
| Trace element solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.949 | - | |
| Na2S2O3 x 5 H2O | 3.16 | g | 2.998 | 12.08 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.474 | - | |
| Resazurin | 0.50 | mg | 4.7e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.2 and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 8%) |
25.00 | ml | - | - | |
|
Sodium lactate
(1 M) |
20.00 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere). | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Media with this solution
| J953 | IRD DESULFOCURVUS MEDIUM |