Main sol. J957
Internal Solution-ID: S4979
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.0 | g | 99.206 | 1697.58 | |
| MgSO4 x 7 H2O | 10.0 | g | 9.921 | 40.25 | |
| KCl | 6.0 | g | 5.952 | 79.844 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.397 | 2.699 | |
| NH4Cl | 1.0 | g | 0.992 | 18.547 | |
| KH2PO4 | 0.1 | g | 0.099 | 0.729 | |
| Yeast extract | 0.5 | g | 0.496 | - | |
| FeCl2 solution | 1.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
| Resazurin | 0.5 | mg | 5.0e-4 | 0.002 | |
| Distilled water | 890.0 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized) to the medium: | |||||
|
Trace vitamins
(*) |
10.0 | ml | - | - | |
|
NaHCO3
(*, 8%) |
50.0 | ml | - | - | |
|
L-Sodium lactate
(5%) |
50.0 | ml | - | - | |
| 2 Finally, add per liter the following solution from an anaerobic stock: | |||||
|
Na2S x 9 H2O
(5%) |
6.0 | ml | - | - | |
Media with this solution
| J957 | DESULFOHALOBIUM UTAHENSE MEDIUM |