Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Bacto peptone
(BD-Difco) |
10.0 | g | 9.804 | - | |
| Urea | 2.0 | g | 1.961 | 32.649 | |
| K2HPO4 | 2.5 | g | 2.451 | 14.072 | |
| KH2PO4 | 3.0 | g | 2.941 | 21.613 | |
| NaCl | 3.0 | g | 2.941 | 50.328 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.196 | 0.796 | |
| Trace vitamins | 10.0 | ml | - | - | |
| Trace element solution | 10.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 8.0. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. | |||||
Media with this solution
| J967 | KD-1 MEDIUM |