Main sol. J978
Internal Solution-ID: S5005
Equipment needed: Filter
Autoclave
Anaerobic workbench
| 1 Mix components thoroughly and adjust pH to 7.25 with NaOH. Distribute the medium into culture vessels (e.g., 5.0 ml in 25 ml serum bottles/Balch tubes) under a stream of N2, seal with butyl rubber stoppers and autoclave. After cooling, aseptically add per liter the following solutions (autoclaved or *filter-sterilized and stored anaerobically): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 3.000 | g | 2.899 | 49.599 | |
| KCl | 0.150 | g | 0.145 | 1.944 | |
| Na2SO4 | 0.300 | g | 0.29 | 2.041 | |
| MgSO4 x 7 H2O | 0.123 | g | 0.119 | 0.482 | |
| CaCl2 x 2 H2O | 0.015 | g | 0.014 | 0.099 | |
| NH4Cl | 0.107 | g | 0.103 | 1.933 | |
| NaH2PO4 | 1.380 | g | 1.333 | 11.113 | |
| Mineral solution (see Medium No. 976 | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
|
Sodium acetate
(0.2 M) |
10.000 | ml | - | - | |
|
Sodium Thiosulfate
(0.2 M) |
10.000 | ml | - | - | |
|
NaHCO3
(w/v, *, 8.0%) |
10.000 | ml | - | - | |
| 2 Before inoculation, add filter-sterilized O2 gas or air, at a final concentration of 2% O2 in the gas phase. | |||||
Media with this solution
| J978 | THERMOCRINIS JAMIESONII MEDIUM |