Main sol. J983
Internal Solution-ID: S5012
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 80.0 | g | 75.901 | 1298.79 | |
MgSO4 x 7 H2O | 25.0 | g | 23.719 | 96.233 | |
CaCl2 x 2 H2O | 4.5 | g | 4.269 | 29.041 | |
KCl | 1.5 | g | 1.423 | 19.09 | |
FeCl2 solution | 1.0 | ml | - | - | |
Trace element solution | 1.0 | ml | - | - | |
Selenite-tungstate solution | 1.0 | ml | - | - | |
Resazurin | 0.5 | mg | 4.7e-4 | 0.002 | |
Pullulan | 0.5 | g | 0.474 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized and stored under N2): | |||||
NH4Cl
(1%) |
10.0 | ml | - | - | |
KH2PO4
(1%) |
5.0 | ml | - | - | |
Trace vitamins
(*) |
10.0 | ml | - | - | |
Na2CO3
(5%) |
20.0 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.0 | ml | - | - | |
2 Adjust pH to 7.2 - 7.5 with a 5% Na2CO3 solution, if necessary. |
J983 | ARTIFICIAL SEAWATER MEDIUM WITH PULLULAN |